The objects of molecular genetic studies were in vitro cultures, trees from the artificial and natural stands of Betula pendula Roth.var. carelica Mercl., Betula pubescens Ehrh. and Betula pendula Roth. The phylogenetic relationships between these species were investigated using a part of the nuclear ADH gene sequences. The ADH phylogeny suggests existing systematics of curly birch. Five polymorphic microsatellite loci were used for additional genetic analysis of forms of Betula pendula var. carelica. Results of study showed features of genetic structure of habitual forms by isozyme and SSRP assay.